Aswir AR and Wan Nazaimoon WM (2011). Effect of edible bird's nest on cell proliferation and tumour necrosis factor alpha (TNF-alpha) release in vitro. International Food Research Journal 18(3): 1073-1077
The human colonic adenocarcinoma cell line (Caco-2 cells) and a macrophage cell line (RAW 264.7 cells) are widely used in vitro model to study the intestinal system and Tumor Necrosis Factor- alpha (TNF-?) release in cells, respectively. The objectives of this study were to assess the proliferative effect of EBN on Caco-2 cells and its effect on TNF-? release in RAW cells. The percentage of cell proliferation when treated with 2 commercial EBN, brand Y1 and brand X1, were 84% and 115% respectively, while when treated with unprocessed EBN from East Coast, North and South Zones were 91%, 35% and 47% respectively. Several EBN from specific zones (brand Y1, South and East Coast Zones) significantly affect the TNF-? production in RAW cells, where it was reduced to 43%, 24% and 32% respectively. Results showed that depending on the source and type of EBN used, there were differences in the percentages of proliferation of Caco-2 cells. EBN is able to influence the production of anti-inflammatory TNF-? in RAW cells.