[ English | Bahasa Malaysia ] Hari ini ialah 26 Oct 2020, 08:39 AM (Terakhir dikemaskini pada: 22nd Oct 2020)

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IDRC : Virology

. Terakhir dikemaskini: 29 Januari 2018Jumlah paparan: 21298

 

The Virology Unit continued to be the main Public Health Laboratory in the Ministry of Health providing diagnostic services for the prevalent medical viral infection, serving most government hospitals and clinics in the country. It also serves as reference laboratories for diagnosis of wide range of diseases. These include:

  • WHO National Influenza Centre
  • WHO National Laboratory for Poliovirus
  • WHO National Japanese Encephalitis Laboratory
  • National AIDS Reference Laboratory
  • National Laboratory for potency testing of Viral Vaccines viz Measles, Polio live viral vaccines
  • National Reference Laboratory for SARS Corona Virus
  • National Reference Laboratory for Avian Influenza

 

Statistical overview

 

NUMBER OF LABORATORY SERVICES PERFORMED BY

VIROLOGY UNIT (2001 – 2010)

ViroPicture1

 

SEROLOGICAL TESTS PERFORMED BY THE VIROLOGY UNIT IN 2010

Virofraf2

 

SPECIALISED TEST: VIRAL ISOLATION & IDENTIFICATION PERFORMED IN 2010

Virograf3

 

SPECIALISED TEST: MOLECULAR TECHNIQUES PERFORMED IN 2010

Virograf4

 

All diagnostic work in the unit is carried out in 3 main laboratories namely molecular diagnostic, serology and tissue culture. The list of current tests performed at the unit is as follows:

 

Serology

  • Dengue (Haemagglutination Inhibition & ELISA-IgM)

  • Japanese encephalitis (Haemagglutination Inhibition & ELISA-IgM)

  • Nipah(ELISA-  IgM &IgG)

  • Rubella (ELISA-  IgM &IgG)

  • CMV(ELISA-  IgM &IgG)

  • HIV (ELISA, Particle Agglutination, Immunoblot, Western blot)

  • Hepatitis Types A, B & C

 

 Viro1   ViroPic2

  ELISA for detection of Japanese Encephalitis

  Haemagglutination Inhibition Test

 

Tissue Culture

 

  • Viral Meningitis/Encephalitis/ Cardiomyopathy Syndrome
    • Cell Culture
    • Neutralization Assay
    • Immunoflurorescent Ab assay

 

  • Hand, Foot & Mouth Disease
    • Cell Culture
    • Neutralization Assay
    • Immunoflurorescent Ab assay

 

  • CMV Reference Test
    • DEAFF
    • Cell Culture
    • Neutralization Assay

 

  • Poliomyelitis
    • Cell Culture of AFP (Acute Flaccid Paralysis) and Non- AFP specimens
    • Typing by Microneutralization Assay
    •  Immunoflurorescent Ab assay
    •  Intratypic Differentiation (ITD)
    •  Vaccine Derived Poliovirus

 

  • Arbovirus Isolation Work
    • Cell culture
    • Suckling mice
    • Immunofluorescence Ab assay

 

  • Chikungunya and Influenza  viruses
    • Cell Culture
    • Immunoflurorescence Ab assay

 

  • Rabies

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 ViroPic3Handling of risk group 3 viruses in BSL 3 Lab

 ViroPic4Tissue culture work in BSL 2 laboratory
 ViroPic5Uninfected MDCK cell culture
 ViroPic6Infected MDCK cell culture
 ViroPic7Immunoflurorescence Ab assay

 

Molecular Diagnostics

List of reference tests performed in the unit by molecular techniques

  • Pan-Enterovirus
  • EV-71
  • Dengue
  • JE
  • Chikungunya
  • HIV
  • SARS CoV
  • Human Influenza A / B, Avian Influenza
  • Outbreak H1N1 PCR Tests
  • A (H1N1)Oseltamivir Resistant Tests
  • Pandemic H1N1 mutation analysis by sequencing

 

ViroPic8     ViroPic9     ViroPic10     ViroPic11

Personnel opreating the PCR thermal cycle

 

Real Time PCR Thermal cyclers in the unit  

Preparation of master mix for PCR using liquid handling system

 

Virology Unit’s Sequencer 3730xl

 

Outbreak Investigations

Examples:

  • SARS in 2003
  • Avian Influenza investigations: 2004, 2006 & 2007
  • Chikungunya from 2008 to 2009
  • Recent influenza A (H1N1) pandemic in 2009

 

ViroPic12 ViroPic13 ViroPic14
Handling of specimens during outbreaks

 

ViroPic15

Negative stained  adenovirus particles, 80 nm diameter from infected Hep-2 culture fluid  isolated from an acute respiratory disease outbreak in a police training centre in Kuala Lumpur 2011 (Bar=100nm)

 

ViroPic16 ViroPic17

Negative stained  particles Influenza B virus particles in respiratory specimens from acute respiratory disease outbreak in Penang 2006.

 

ViroPic18 ViroPic19

Severe cytopathological effect (CPE) from Chikungunya virus infection results in rupture of plasma membrane as newly assembled virus bud out of the cell (Chikungunya Outbreak 2008-2009).

 

ViroPic20 ViroPic21

Electron micrograph  shows  cluster of negatively stained rotavirus  particles in stool specimens of infected patients  in the acute gastroenteritis outbreak in April 2004 in Cameron Highlands, Pahang.   Bar = 100 nm.

myGOV MAMPU JPA IKU MSC NMMR CRC MyHEALTH MOH Globinmed SPA krste.my

Notis

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