[ English | Bahasa Malaysia ] Hari ini ialah 3 Aug 2020, 10:28 PM (Terakhir dikemaskini pada: 27th Jul 2020)

Text Red Text Black Text Blue Text Green Text Smaller Text Reset Text Larger

Publication Details
Year :

2015

Journal :

Umapathy T, Alias R, Lim MN, Alagaratnam J, Zakaria Z and Lim TO (2015). Clinical outcome of autologous cultivated limbal epithelial transplantation therapy for patients with ocular surface disease. Stem Cell Research and Therapy 4:11

Abstract :

Introduction: Cultivated Limbal Epithelial Cells Transplantation (CLET) has been proven effective in reconstructing cornea surface of Limbal Stem Cell Deficiency (LSCD). To better understanding the potential and risk of this treatment in our current setup, we conducted a phase I clinical trial and determined the outcome of cultivated limbal epithelial transplantation for Ocular Surface Disease (OSD).

Methods: Prospective interventional trial was conducted in Hospital Kuala Lumpur, Malaysia. Fourteen eyes of 14 patients with limbal stem cells deficiency: chemical injury (6), advanced pterygium (4), advanced Vernal Kerato Conjunctivitis (VKC) (2), Persistent Epithelial Defect (PED) (1) and Ocular Cicatricial Pemphygoid (OCP) (1) were selected according to the inclusion/exclusion criteria. Autologous cornea limbal epithelial stem cells were cultured on human amniotic membrane and subjected to immunohistological staining for a panel of markers (ABCG2, cytokeratins (K) 3, K19, p63, involucrin, integrin α9 and K14). Cell sheets with epithelial morphology and growth area greater than 80% will be used for CLET. Two weeks later, the tissue was transplanted to the recipient eyes after superficial keratectomy. The patients were followed up for 1 year. Outcome measures were improvement of symptoms, an improvement in visual acuity, no conjunctivalisation and vascularisation and healed persistent corneal epithelial defect.

Results: Ten patients (71.4%) had an improvement in visual acuity of at least two lines at 6 months and one year (p<0.05%). Thirteen patients (92.8%) had cornea epithelisation in 1 month. The patient with persistent epithelial defect (PED) healed in 3 months and the patient with ocular cicatricial pemphygoid (OCP) had recurrent cornea epithelial defect at 6 months and healed after one year. On slit lamp examination, eight patients (57.1%) had no cornea vascularisation in 6 months and 1 year (p<0.05%). Ten patients (71.4%) had no cornea conjunctivalisation in 6 months and 1 year (p<0.05%).

Conclusions: Transplantation of cultivated autologous limbal epithelial cell can efficiently restore the cornea with improved vision. Patients with chemical injury, advance pterygium group had better outcome results.

myGOV MAMPU JPA IKU MSC NMMR CRC MyHEALTH MOH Globinmed SPA krste.my

Notis

Logo-SIRIM-n-UKAS-IMR

IMR ialah sebuah organisasi bertauliah oleh SIRIM (nombor sijil 2972).

Laman ini sesuai dilihat menggunakan Firefox (24.0) dengan resolusi 1280 x 768 pixels atau lebih.

Lokasi Kami

world

Kami terletak di Jalan Pahang, 50588 Kuala Lumpur, Malaysia. Kami beroperasi dari jam 8.00pg - 5.00ptg Isnin - Jumaat. Klik pada imej peta untuk melancarkan servis Google Maps.

Hubungi Kami