[ English | Bahasa Malaysia ] Hari ini ialah 3 Aug 2020, 11:11 PM (Terakhir dikemaskini pada: 27th Jul 2020)

Text Red Text Black Text Blue Text Green Text Smaller Text Reset Text Larger

Publication Details
Year :

2012

Journal :

Noraziah Nordin, Juriyati Jalil, Ibrahim Jantan and Shahnaz Murad (2012). Platelet-activating factor (PAF) receptor binding activity of the roots of  Enicosanthellum pulchrum. Pharmaceutical Biology 50(3): 284-290

Abstract :

Context: Enicosanthellum pulchrum (King) Heusden (Annonaceae) is a coniferous tree that is confined to mountain forests. The chemical constituents of this species have been studied previously; however, its biological activity has never been investigated before and is reported here for the first time.

 

Objective: The extracts, fractions and compounds from the roots of E. pulchrum were investigated for their inhibitory effects on platelet-activating factor (PAF) receptor binding to rabbit platelets using 3H-PAF as a ligand.

 

Materials and methods: The PAF receptor binding inhibitory effect using rabbit platelets was determined in vitro by measuring the difference between total amount of bound 3H-PAF in the presence and the absence of excess unlabelled PAF. The compounds were isolated by bioassay-guided fractionation and their structures were elucidated by spectroscopic techniques.

 

Results and discussion: Among the extracts tested, the ethyl acetate extract was the most active with 85.6% inhibition, while hexane and methanol extracts showed 40.2 and 42.5% inhibition, respectively. Fractionation of the ethyl acetate extract using vacuum liquid chromatography (VLC) yielded six fractions AEA(I-–VI). Chromatography fraction AEA(VI) yielded a new compound, 1-(2′,3′,4′-trimethoxyphenyl)hexan-1-ol, while fraction AEA(III) afforded three compounds, namely liriodenine, cleistopholine and dehydroanonaine. 1-(2′,3′,4′-Trimethoxyphenyl)hexan-1-ol, cleistopholine and dehydroanonaine showed relatively strong inhibition with IC50 values of 26.6, 50.2 and 45.4 µM, respectively.

 

Conclusion: The results suggest that these compounds could be responsible for the PAF antagonistic activity of the ethyl acetate extract of this plant.

 

 

 

myGOV MAMPU JPA IKU MSC NMMR CRC MyHEALTH MOH Globinmed SPA krste.my

Notis

Logo-SIRIM-n-UKAS-IMR

IMR ialah sebuah organisasi bertauliah oleh SIRIM (nombor sijil 2972).

Laman ini sesuai dilihat menggunakan Firefox (24.0) dengan resolusi 1280 x 768 pixels atau lebih.

Lokasi Kami

world

Kami terletak di Jalan Pahang, 50588 Kuala Lumpur, Malaysia. Kami beroperasi dari jam 8.00pg - 5.00ptg Isnin - Jumaat. Klik pada imej peta untuk melancarkan servis Google Maps.

Hubungi Kami