Jama'ayah Mohamed Zahidi, Tay Bee Yong, Rohaidah Hashim, Azura Mohd Noor, Siti Hawa Hamzah and Norazah Ahmad (2015). Identification of Brucella spp. Isolated from human brucellosis in Malaysia using High-melt resolution (HRM) analysis. Diagnostic Microbiology and Infectious Disease 81(4): 227-233
Molecular approaches have been investigated to overcome difficulties in identification and differentiation of Brucella spp. using conventional phenotypic methods. In this study, high-resolution melt (HRM) analysis was used for rapid identification and differentiation of members of Brucella genus. A total of 41 Brucella spp. isolates from human brucellosis were subjected to HRM analysis using 4 sets of primers, which identified 40 isolates as Brucella melitensis and 1 as Brucella canis. The technique utilized low DNA concentration and was highly reproducible. The assay is shown to be a useful diagnostic tool, which can rapidly differentiate Brucella up to species level.