Research activities in Phytochemistry Unit are directed towards the development of value added and safe standardized extracts and discovery of new molecule entities from natural resources with the focus on cancer, malarial, dengue, and neglected tropical diseases. Activities include field collection, extraction, chromatographic and spectroscopic evaluation, isolation and structural elucidation of bioactive metabolites.
The bioassay guided fractionation approach used for the natural products drug discovery are conducted in collaboration with various research establishments within and outside the Institute. More than 100 extracts and fractions have been deposited in the unit repository and are accessible for further studies. Similarly, structural elucidation of isolated bioactive compound using state of the art spectrometry technology in particular the Mass Spectrometry (MS), Nuclear Magnetic Resonance (NMR) and Fourier-Transform Infrared (FT-IR) are performed through collaborative efforts.
The unit is also committed in studies related to chemical profiling and characterization of medicinal plant and fungi extracts for a range of secondary metabolites as well as carbohydrate molecules using new approaches and methodologies for the development of standardize products.
- The capabilities of the Unit in medicinal research are supported by various laboratories facilities:
- Drying and concentration equipment; Rotary Vacuum Concentrator, Rotary Evaporator, Spray Dryer and Freeze Dryer
- Chromatography and spectroscopic evaluation: UV-vis spectrophotometer, HPLC-DAD, HPLC-RI, GCMS and automated HPTLC/TLC system
- Separation of complex mixtures; Semi-preparative HPLC-UV and Flash chromatography with various stationary phases.