Mansor Fazliana, Harvest F Gu, Claes-Goran Ostenson, Mashitah Mohd Yusoff and WM Wan Nazaimoon (2012). Labisia pumila extract down-regulates hydroxysteroid (11-beta) dehydrogenase 1 expression and corticosterone levels in ovariectomized rats. Journal of Natural Medicines 66(2): 257-264
We evaluated the effects of a standardized Labisia pumila var. alata (LPva) extract on body weight change, hydroxysteroid (11-beta) dehydrogenase 1 (HSD1 1B1) expressions and corticosterone (CORT) level in ovariectomized (OVX) rats. The decoction of LPva has been used for generations among Malay women in Malaysia to maintain a healthy reproductive system. Thirty-six Sprague–Dawley OVX rats were treated orally with LPva extract (10, 20 or 50 mg/kg/day) or estrogen replacement (ERT) for 30 days. Sham operated rats were used as controls. Compared to untreated OVX rats, LPva-treated rats showed less weight gain and had signiﬁcantly down-regulated HSD11B1 mRNA in liver tissues. HSD11B1 mRNA in adipose tissues increased by 55% (p<0.05) in OVX rats but normalized in rats treated with LPva. Similarly, there was signiﬁcant down-regulation (p<0.05) of protein levels of HSD11B1 in both liver and adipose tissue of LPva and ERT groups ,and CORT levels were signiﬁcantly reduced in both groups of rats. This is the ﬁrst study ever conducted to evaluate the beneﬁcial effects of LPva in relation to weight gain caused by estrogen insufﬁciency. Results implied that the bioactive components in LPva extract affect not only HSD11B1 expressions in both adipose and liver tissues but also decrease circulating CORT. The extract should be explored for its potential use as a natural remedy for weight management.