Entomology Unit
| Introduction | Research | Diagnostic Services |
RESEARCH
| 2005 | 2004 | 2002 | 2001 | 2000 | 1999 | 1998 |
Year 2005 
Malaria Vectors
Comparative field evaluation of residual-sprayed deltamethrin WG and deltamethrin WP for the control of malaria in Pahang, Malaysia (
Rohani A, Lee HL, Rahini H, David L, Kamilan D
)
Dengue Vectors
Field bioefficacy of a Bacillus thuringiensis H-14 formulation (VectoBac® WG) for the control of dengue vectors (
Lee HL, Seleena P, Chen CD, Masri M, Chiang YF).
)
Effects of sublethal dosages of an insect growth regulator (pyriproxifen) on dengue susceptibility of Aedes aegypti (
Lee HL, Lau VI, Rohani A, Zamree I
)
Temperature related storage evaluation of an RT-PCR test kit for the detection of dengue infection in mosquitoes (
Ooi CP, Rohani A, Zamree I, Lee HL
)
Detection of transovarial dengue virus in field-caught Aedes aegypti and Aedes albopictus mosquitoes using C6/36 cell culture and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques (
Rohani A, Zamree I, Lee HL, Mustafakamal I, Norjaiza MJ, Kamilan D
)
Laboratory study on the persistency of transovarial dengue virus in Aedes aegypti (
Lee HL, Joseph RT, Rohani A, Zamree I
)
Sensitivity of Aedes albopictus C6/36 cells line for the detection and infectivity titration of dengue virus (
Rohani A, Zamree I, Drakes ON
)
Field bioefficacy of deltamethrin residual spraying against Aedes vectors (
Rozilawati H, Lee HL, Masri M, Mohd Nor, Rosman S
)
Laboratory and field evaluation of copper wire on Aedes albopictus larval development (
Vythilingam I, Norzawati Y
)
Preliminary study on the field efficacy of the Mosquito Slayer®, a mosquito trapping device (
Wan Norafikah O, Lee HL, Sa'diyah I, Chen CD, Chan ST , Ong YF, Wan Norjuliana WM, Nor Azlina AH
)
Chikungunya Vector
Rapid detection of chikungunya virus in laboratory infected Aedes aegypti by Reverse-Transcriptase- Polymerase Chain Reaction (RT-PCR) (
Rohani A, Yulfi H, Zamree I, Lee HL
)
Other Mosquitoes/Studies
A comparative study of adult mosquito trapping using dry ice and yeast generated carbon dioxide (
Vythilingam I, Oli K, Jeffery J
)
Other Insects
Biocompatibility testing of Pycnoscelus cockroach: Primary skin irritation and skin sensitization study (
Khadri MS, Shahnaz M, Milner M, Noor MI
)
Surveillance of diurnal and nocturnal dipterous flies in the Federal Territory of Putrajaya (
Nazni WA , Nooraidah H, Jeffery J, Azahari AH, Lee HL
)
Vespa affinis indosinensis in Peninsular Malaysia (
Lee HL, Krishnasamy M, Jeffery J
)
A case of human nasopharyngeal myiasis caused by Chrysomya bezziana Villeneuve, 1914 (Diptera: Calliphoridae) in Malaysia (
Lee HL, Krishnasamy M, Jeffery J
)
Insecticides Studies
Biological efficacy of commercial household insecticides aerosol spray against Aedes aegypti (Linn.) under simulated field conditions (
Khadri MS, Kwok KL, Noor Halila WMJ, Lee HL
)
Evaluation of residual spraying on sandy beach using pyrethroid Fendona®( a-cypermethrin) in relation to biting midge (Diptera: Ceratopogonidae) control (
Khadri MS, Paulos ST, Abdullah AG
)
Determination of the bioefficacy of insecticide–impregnated net for the control of house flies in a poultry farm ( Nazni WA, Sumarni MG, Lee HL, Abdullah AG, Sa’diyah I, Zamree I, Tan SB, Md Noor I, Azahari AH, Ong YF )
Biochemical analysis of insecticide susceptibility in Culicine mosquitoes in malaria endemic areas (
Rohani A, Nazni WA, Luxana L, Saadiyah I, Lee HL
)
Year 2004
Microbial Control agents and other biological control agents field
Bioefficacy of a Bacillus thuringiensis H-14 tablet formulation against container-breeding Aedes aegypti & Ae albopictus (
Seleena B, Andrew R, Chiang YF, Lee HL
)
Evaluation of VectoBac® WG for dengue vector control in Malaysia (
Lee HL, Chen CD, Masri M, Chiang YF and Seleena B
)
Insecticides Studies
Temporal effect of pyrethroid formulations applied by ULV generator against the housefly, Musca domestica (
Nazni WA , Siti Salina, Lee HL
)
Field evaluation of diflubenzuron, a urea-based chitin synthesis inhibitor against container-breeding Aedes (
Lee HL, Seleena P, Chiang YF, Chen ZD
)
Malaria Vectors
A longitudinal survey to determine malaria epidemiology and vector distribution in Attapeu province, Lao PDR (
Vythilingam I, Sidavong B, Chan ST et al )
Adulticidal effect of deltamethrin water dispensable granule (WG) formulation against malaria vector Anopheles maculatus mosquito on bamboo surfaces (
Rohani A, Arthur S,Abdullah AG, Saadiyah I, Lee HL
)
Field evaluation of residual-sprayed deltamethrin WG formulation against malaria vector (
Lee HL, Rohani A, Bayer Environmental Science
)
Dengue Vectors
Laboratory and field evaluation of the insect growth regulator pyriproxyfen (Sumilarv 0.5G) against dengue vectors (
Vythilingam I, Belleza ML, Tan CH
)
Infection proteins in dengue-infected Aedes aegypti (
Wong YC, Rohani A, Zamree I, Lee HL
)
Rapid detection of dengue virus in field caught Ae. aegypti and Ae. albopictus using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) (
Rohani A, Lean KS , Zamree I, Lee HL
)
Detection of dengue virus from field Aedes aegypti and Aedes albopictus larvae collected from Terengganu (
Rohani A, Mustafakamal I, Zamree I, Lee HL
)
Development of rapid PCR test kit for dengue virus detection in mosquitoes (
Rohani A, Ooi CP, Zamree I, Lee HL )
Effects of ultra-violet light C on the dengue vectorial capacity of Aedes aegypti and Aedes albopictus (
Lee HL, Rohani A, Zamree I
)
Other Mosquitoes/Studies
Mosquito larval survey in different habitats in Putrajaya (
Nazni WA , Luisa N, Lee HL and All Medical Entomology Support Staff
)
Mosquito preferences for membrane of different animal origin ( Ooi CP, Lee HL, Rohani A, Zamree I )
Other Insects
Maggot therapy of intractable wounds (
Lee HL, Nazni WA, Rogayah, Subramaniam S
)
Extraction of anti-microbial agent(s) from larvae of Lucilia cuprina (
Lee HL, Nazni WA , Seleena P
)
Protein profiles of fly larvae & application in forensic entomology (
Lee HL, Nazni WA, Phasouk S, Azahari H
)
Biting midges in coastal beaches (
Khadri MS, Entomology Staff
)
Flea survey and rat control (
Lee HL, Krishnasamy M
)
Year 2002
Microbial control of Japanese Encephalitis vectors in Serian District, Sarawak – pilot study (Seleena B, Lee HL, David L, Asmad M & Sarawak VBDCP)
Tablet formulation of Bacillus thuringiensis H-14 for the control of Aedes mosquitoes in potable water containers (Seleena P, Chiang YF, Lee HL & Valent Biosciences)
Lab evaluation of bioefficacy of Bacillus thuringiensis H-14 & sphaericus against Chironomus larvae (Jessica CSK, Seleena P & Lee HL)
Chironomid larval control in oxidation ponds using Bacillus thuringiensis H-14 (Seleena P, Lee HL, Chiang YF, Subramaniam S, DB Cheras & Valent Biosciences)
Effects of sub-lethal dosages of Bti on susceptibility of Aedes aegypti to dengue infection
Anti-cancer effects of Malaysian isolates of Bacillus thuringiensis (lee HL, Vishna D & Seleena P)
Cloning of toxin genes of Clostridium bifermentans serovar Malaysia (Sarjeet S, Seleena P & Lee HL)
Toxicity, morphogenetic and histopathological effects of insect growth regulators in maggot of Musca domestica L ( Nazni WA , Chooi SH, Azimah & Lee HL)
Laboratory & field evaluation of MMF against container-breeding Aedes mosquitoes (Lee HL, Seleena P & Chiang YF)
Field evaluation of pyrethroid formulations against mosquitoes ((Hoan VN, Lee HL, Seleena P & Chiang YF)
Laboratory evaluation of repellent formulation of DEET and natural plant product (Siv S & Lee HL)
Effect of insecticide resistence on the reproductive potential and longevity in vector mosquitoes ( Nazni WA , Choh FH & Lee HL)
Effect of insecticide resistence on development of dengue virus in Aedes aegypti (Monginsidi, Lee HL, Nazni WA , Hidayati H & Rohani A)
A longitudinal survey to determine malaria epidemiology and vector distribution in Attapeu Province , Lao PDR (Vythilingam I, Bounpone, Rattanaxay, Chan et al )
A trial on the effect of insecticide impregnated nets on (a) vector population & (b) malaria parasite rate of population in Sabah (Vythilingam I, Shanmugaratnam C, Chooi CK, Chan ST et al )
Field trial to determine the efficacy of pyrethroid insecticide application using ultra low volume for the control of Aedes mosquitoes (Vythilingam I, Tanrang Y, Tan & Abdullah G)
Protein synthesized by Aedes aegypti and Aedes albopictus mosquito in response to dengue virus infection (Rohani A, Hidqayat T, Zamree I & Lee HL)
Study on leptoconine sandflies in resort islands (Khadri MS, Lee HL & Johore VBDCP)
Close-up view of Leptoconops spinosifrons biting midge found at Pulau Besar Johor island. The intense itchy bite had causing some impact to tourism industry. |
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The larvae of Leptoconops sp. emerging from the eggs - hatching.
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The first IMR trip to Pulau Aur in March 2002, by a fishing boat. It was a 6 hour journey from Mersing jetty to the island. Leptoconops were found at the beaches.
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Control activities against Leptoconops sp in Johore islands. The boat was heavily loaded with fogging and spraying equipment and with personnel. It was a collaboration with the Public Health Department of Johor e and the Marine Police. This was towards the end of March 2002.
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A boat was rented for the study on biting midges at some beaches on Pulau Aur and Pulau Dayang islands. This was in early March 2002. |
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An overview of Pulau Aur showing a fishing village with crystal clear sea-waters. This was in early March 2002.
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A photograph showing some cows which may a contributing factor to the high number of biting midges at some of the islands off Johore. A preliminary study showed that there were no Leptoconops found biting the cows; neither were they detected in cow dung. |
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An Entomology staff was bitten by a biting midge. The itchiness lasted up to 3-4 weeks.
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A photograph which shows a biting midge having a blood meal. |
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A scenery of a work area at a beach at one of islands in Langkawi. No Leptoconops sp was found at the island.
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Field evaluation of commercial aerosols against leech (Lee HL, Krishnasamy M & John J)
Production of sterile fly maggots for wound therapy (Lee HL, Rogayah, Nazni WA & Seleena P)
Year 2001
Mosquitocidal activity of Clostridium bifementas isolates (Seleena P, Cheong CL & Lee HL)
Laboratory studies on fermentation of Bacillus thuringiensis Pahangi (H-69) (Seleena P, Wong KM & Lee HL)
Pilot plant pre-commercial production of a Malaysian Bacillus sphaericus isolate (lee HL, Inbiotech Sdn Bhd & Seleena P)
Thermal application of Bacillus sphaericus (Lee HL, David Lubim & Seleena P)
Microalgae associated with mosquito breeding grounds in Malaysia and their potential use as biocontrol agents (Rohani A, Zamree I, Phang SM, Chu WL, Nicholas K & Lee HL)
Effects of pyrethroud formulations against sandflies (Khadri MS, Lokman Hakim S, Lee HL & Seleena P)
Bioefficacy of repellents (Lee HL, Nazni WA, Azahari H, Sadiyah I & Hidayati H)
Development of comprehensive test kits for the rapid detection of insecticide resistance due to various mechanisms (lee HL, Vykhodtsev A, Nazni WA & Sadiyah I)
A trial on the effect of insecticide impregnated nets on vector population and malaria parasite rate of a population in Sabah (Vythilingam I, Shanmugaratnam C, Chooi CK, Chan ST, Lokman SH & Abdullah G)
Epidemiology of malaria in relation to entomological parameters in Sekong Province , LAO PDR – The entomological picture (Vythilingam I, Rattanaxay P, Kalouna K, Lokman HS et al )
Identification of Anopheles minimus and its related species and An. dirus from Sekong Lao PDR using molecular techniques (Vythilingam I, Vilaphanh Y & Tan SB)
Flight range and dispersal of Aedes aegypti and Ae. albopictus (Lee HL, Seleena P, Chiang YF, Subramaniam S & Krishnasamy)
Protein synthesized by mosquito in response to dengue virus infection (Rohani A, Yunus W, Zamree I & Lee HL)
Rapid detection of dengue virus in mosquito using dipstick (Vythilingam I, Purwanto, Ravindran T & Zainol Pawanchik)
Susceptibility status of Culex sitiens to Japanese encephalitis virus in Peninsular Malaysia (Vythilingam I, Tan SB & Krishnasamy)
Sand flies bionomics studies (Khadri MS, Anif B & Lee HL)
Effect of time of insecticide application on the susceptibility of house fly, Musca domestica ( Nazni WA , Davaa Gombojav, Lee HL & Sa'diyah I)
Year 2000
Isolation of novel mosquitocidal bacteria (Seleena P, Daniel Duval, Lee HL, Gaudio N, Little S, Andrew Rath & Kokkinn M)
Effect of four chlorophytes on larval survival, development and adult body size of the mosquito Aedes aegypti (Rohani Ahmad, Chu WL, Lee HL & Phang SM)
Large scale fermentation studies on a Malaysian isolate of Bacillus sphaericus ( IMR-BS-4 ) (Lee HL & Inbiotech Sdn Bhd)
Field evaluation of MOSPHERIX, a Malaysian Bacillus sphaericus product (Lee HL, Kapa & Seleena P)
Laboratory and field trials on the efficacy of commercial repellents against mosquitoes of public health importance (Lee HL, Vythilingam I, Nazni WA, Latipah Omar & the Division of Entomology staff)
Evaluation of Solfac, alpha-cypermethrin and deltametrin treated nets against mosquitoes of public health importance under field conditions (Vythilingam I, Sutjipto & Tan SB)
Evaluation of a pyrethroid formulation applied by cold and thermal fogging (Lee HL, Long VT, Seleena P & Chiang YF)
Laboratory evaluation of commercial household aerosol (Lee HL, Seleena P, Nazni WA, Sadiyah I, Zambree & Chiang YF)
Development of comprehensive rapid tests for the screening of insecticide resistance in dedically important insects (Nazni WA, Raweewan S & Lee HL)
Control of malaria due to Anopheles balabacensis in Sabah (Lee HL, Seleena P & Chooi KH)
Bionomics of Aedes mosquito as a dengue vector (Eow GH & Lee HL)
Effects of ribavirin and hydroxyureaq on the development of dengue virus in Aedes aegypti adults (Lee HL, Tran VP & Rohani A)
Colonisation of Mansonia uniformis using various paper materials (Lee HL, Amos M & Abdullah G)
Susceptibility of Culex sitiens to Japanese encephalitis virus in Peninsular Malaysia (Vythilingam I, Tan SB & Krishnasamy)
Sandfly studies (Khadri MS & Lee HL)
Phlebotomus sp sand fly is indigenous in Malaysia. This sand fly is the main vector for leishmaniasis in India, Bangladesh, Sudan, etcetera. No indigenous case has been reported in Malaysia. |
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A renovated cave which serves as a temple at Ipoh, Perak, where a sand fly survey was carried out.
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A limestone cave was identified as a main habitat of the Malaysian sand fly. Two personnel were photographed fixing light traps at Gua Musang, Kelantan. |
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A recreational park in a limestone area at Ipoh, Perak was also surveyed for sand flies.
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The Ipoh Society for the Protection Against Cruelty to Animals (SPCA) is located in a cave in Ipoh town. A sand fly survey was conducted by the road side. |
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This photograph shows some Entomology staff among Bangladeshis at the Cameron Highlands tea plantation. No sand-fly was found in the survey.
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A photograph showing the comparative sizes of Aedes aegypti, Culicoides and Phlebotomus. |
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Bionomics of Culex quinquefasciatus , vector of urban bancroftian filariasis (Nazni WA, Anthony LT, Lee HL, Ong YF, Abdullah G & Sa'diyah I)
Flight range determination studies on the house fly, Musca domestica using fluorescent dusting technique ( Nazni WA , Luke H, Lee HL, Sa'diyah I & Abdullah G)
Year 1999
Field evaluation of VECTOLEX ® , Bacillus sphaericus against Culex (Lee HL, Seleena P & Samreng P)
Fermentation studies on Malaysian isolate of Bacillus sphaericus (Lee HL & Inbiotech Sdn Bhd)
Effect of four chlorophytes on larval survival, development and adult body size of mosquito Aedes aegypti (Rohani Ahmad, Chu WL, Lee HL & Phang SM)
Efficacy of MMF ‹ (MonoMolecular Film) on Culex quinquefasciatus and housefly Musca domestica ( Nazni WA , Lee HL & Sa'diyah I)
Laboratory evaluation of temephos baits for the control of house fly, Musca domestica ( Nazni WA , Lee HL & Sa'diyah I)
Field evaluations of other pyrethroids and organophosphates (Lee HL, Nazni WA , Sadiyah I & Halila)
Effectiveness of an insecticidal paint formulation (Lee HL, Leo Makita, Nazni WA & Sadiyah I)
ULV application of chemical insecticides for the control of housefly, Musca domestica (Nazni WA, Le Thi Dui, Lee HL, Sa'diyah I, Rogayah TAR & Seleena P)
Determination of oxidase activity in relation to insecticide resistence in the mosquito vectors of public heaqlth importance ( Nazni WA , Kamaludin MY, Lee HL & Sa'diyah I)
Rate of resistance development of Culex quinquefasciatus , Aedes albopictus and Aedes aegypti against malathion, permethrin and temephos (Hidayati Hamdan, Nazni WA & Lee HL)
Development and commercialization of a test kit for the rapid detection of insecticide resistance due to elevated level of non-specific esterases (Lee HL & Nazni WA)
Control of malaria due to Anopheles balabacensis in Sabah (Lee HL, Seleena P & Chooi KH)
A comparative study to determine the efficacy of factory and laboratory impregnated bednets (Vythilingam I, Achmad S & Nazni WA)
Ribavirin inhibited development of dengue virus in Aedes aegypti adults (Lee HL, Thornly Nagata Talasaia & Rohani A)
Aedes larval survey in an suburban residential area in Selangor (Nyin LY, Lee HL & Ramachandran CP)
Sandfly studies (Khadri M & Lee HL)
Year 1998
Isolation of novel microbial control agents of mosquitoes (Lee HL, Seleena P & Pasteur Institute, Paris)
Isolates of Bacillus thuringiensis H 14 (Lee HL & Inbiotech Sdn Bhd)
Field evaluation of MOSBAC®, a local product of Malaysian isolate of Bacillus thuringiensis H-14 (Seleena P, Khishigsuren N, Lee HL & Peter Hew)
Thermal fogging of Bacillus thuringiensis H-14 (MOSBAC®) (Lee HL, Seleena P & Chiang YF)
Field evaluation of a pyrethroid formulation dispersed by cold and thermal fogging (Lee HL, Kamilan D, Seleena P & Chiang YF)
Laboratory evaluation of bifenthrin impregnated mosquito nets (Vythilingam I & Barni)
Field effectiveness of a gel bait formulation against domiciliary cockroaches (Khadri MS & Lee HL)
Comparative evaluation of pyriproxyfen and cyromazine for control of house fly Musca domestica ( Nazni WA , Btissam Ameur, Lee HL & Sa'diyah I)
Profiles of resistence enzymes in cockroach treated with hydramethylnon (Khadri MS, Ambicadutt B & Lee HL)
Comparative field evaluation of indoor residual-sprayed deltamethrin and DDT (Lee HL, Tham AS , Mustafa-kamal I & Mastura)
Impact of climate change on malaria transmission in Malaysia (Lee HL)
A highly sensitive, nested polymerase chain reaction based method to detect malaria sporozoites in mosquitoes (Vythilingam I, Nitiavathy K et al )
Aedes surveys in Universiti Putra Malaysia (UPM) (Shahidi J & Lee HL)
Oviposition response of Aedes aegypti mosquito in different types of water (Vythilingam I & Novi S)
Malaysian Phlebotomine sandflies surveillance and identification (Khadri MS, Lee HL, Abdullah AG, Subramaniam S & Zamri I)
Microsatellite polymorphism in Anopheles maculatus from Malaysia (Vythilingam I, Mohd Ludfi & Nitiavathy K)
Production of anti-fly antibodies and their effect on mortality of the house fly, Musca domestica ( Nazni WA , Lee HL & Tengku Rogayah TAR)
Genetic variations in the house fly populations in Peninsular Malaysia ( Nazni WA , T Rogayah TAR, Zamree I & Lee HL)
| Introduction | Research | Diagnostic Services |
