Biotechnology Unit
| Introduction | Research |
| 2005 | 2004 | 2003 | 2002 | 2001 | 2000 | 1999 | 1998 |
RESEARCH
Year 2005 
Lack of association between a deletion polymorphism of the Angiotensin-Converting Enzyme gene and coronary artery disease among our local population (
Normaznah Y, Azizah MR, Rosli MA, Saniah K, Kuak SH
)
Study of trophozoite surface protein (TSA 11) gene polymorphism in local isolates of Giardia duodenalis using DNA sequencing techniques (
Latifah I, Teoh KY , Wan KL, Rahmah M, Normaznah Y, Rohani A
)
Interleukin-1 beta gene polymorphism in systemic lupus erythematosus (
Azizah MR, Kuak SH, Norella K, Normaznah Y
)
Year 2004
The present main areas of research carried out by the Unit incluse:
Legionella pneumophila - PCR amplification and DNA sequencing of the macrophage infectivity (mip) gene
Systemic Lupus Erythematosus - Studies on the role of Cytokine genes in disease pathogenesis
Giardiasis - A study on Expressed Sequence Tags and Random Length Fragment Polymorphism In
Local Isolates
Salmonella typhi - Monoclonal antibody production
The present research are:
Diagnosis of Legionnaires' disease by urinary antigen and DNA detection (
Normaznah Y, Saniah K, Noor Rain A, Sabiha P, Rahim O
)
Angiotensin-Converting Enzyme gene polymorphism in Chinese patients with Systemic Lupus Erythematosus (
Azizah MR, Kuak SH, Ainol SS, Kong NCT, Rahim MN, Normaznah Y
)
Polymorphism of giardine gene of G. duodenalis (local isolates) using DNA sequencing techniques (
Latifah I, Teoh KY, Rahmah M, Wan KL, Rohani A, Normaznah Y
)
Year 2003
Immunoblt analysis of Legionella pneumophila serogroup 1 antigens using monoclonal and polyclonal antibodies (Normaznah Y, Saniah K, Noor Rain A & Sabiha P)
Lack of association of the IL1-RA gene polymorphism and systemic lupus erythematosus (Azizah MR, Kuak SH, Ainol SS, Rahim MN , Normaznah Y & Norella K)
Role of the TNF-Alpha alleles in genetic susceptibility and pathogenesis of systemic lupus erythematosus (Azizah MR, Kuak SH, Ainol SS, Rahim MN, Normaznah Y & Norella K)
A study on random length polymorphism in local isolates of Giardia lamblia (Latifah I, Teoh KY, Azizah MR, Normaznah Y & Rahmah M)
Prevalence of antibodies to Toxoplasma gondii among farmers and cattle in Gombak District, Selangor, Malaysia (Normaznah Y, Saniah K, Fizina Noor H & Khadijah M)
Year 2002
Increased levels of Interleukin-10 reflect disease activity in patients with Systemic Lupus Erythematosus (SLE) (Azizah MR, Ainol SS, Kuak SH, Kong NCT, Normaznah Y & Rahim MN)
Studies of Expressed Sequenced Tags (ESTs) on local isolates of G. duodenalis (Latifah I, Wan KL, Teoh KY , Normaznah Y & Rahmah M)
Protein profile of Legionella pneumophila serogroup 1 antigen by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunologic response of the immunized rabbit against the antigen (Normaznah Y, Saniah K & Mastuki Y)
Immunoblot analysis of monoclonal antibodies against L. pneumophila serogroup 1 ( Knoxville strain) against homologous and other bacterial antigens (Normaznah Y, Saniah K, Mastuki Y & Anira)
Year 2001
Epitope mapping of monoclonal antibodies against Legionella pneumophila serogroup 1 and cross-reaction with other antigens by immunoelectrotransfer blot (Normaznah Y, Saniah K & Mastuki Y)
Amplification of mip gene of local environmental isolates of Legionella pneumophila serogroup 1 and urine DNA of suspected cases of legionellosis (Normaznah Y, Saniah K & Sabiha P)
Increased levels of Interleukin-10 reflect disease activity in patients with Systemic Lupus Erythematosus (Azizah MR, Ainol SS, Kong NCT, Normaznah Y & Rahim MN)
Class II HLA-DR antigen and DQA, DQB and DPB allele frequencies in Malay patients with Systemic Lupus Erythematosus (Azizah MR, Ainol SS, Kong NCT, Normaznah Y & Rahim MN)
The association of major histocompatibility complex genes and susceptibility to Systemic Lupus Erythematosus among the Malaysian Chinese population (Azizah MR, Ainol SS, Kong NCT, Normaznah Y & Rahim MN)
Construction of cDNA library and expressed sequence tags (ESTs) of local isolate of Giardia duodenalis (Latifah I, Teoh KY , Normaznah Y, Wan KL & Rahmah M)
Year 2000
Determination of the frequency of angiotensin converting enzyme (ACE) gene polymorphism and frequencies of the deletion and insertion alleles among our local population (Normaznah Y, Norhatiah ML, Rosli MA, Azizah MR & Saniah K)
Detection of mutations of b -myosin heavy chain gene and association of the mutation with the angiotensin I converting enzyme polymorphism (Normaznah Y, Nor Shalawati MY, Azizah MR & Rosli MA)
Detection of IgG, IgM and IgA antibodies against Legionella pneumophila serogroup I in serum samples of patients with atypical pneumonia by ELISA (Normaznah Y, Saniah K, Sabiha R, Noor Rain A & Rohani MY)
Demographic, clinical and immunological analysis of 134 Malaysian patients with Systemic Lupus Erythematosus (Azizah MR, Ainol SS, Kong NC, Normaznah Y & Rahim MN)
Clinical and serological disease expression and HLA types in early and late onset disease of Malaysian patients with Systemic Lupus Erythematosus (Azizah MR, Ainol SS, Kuak SH, Kong NCT, Normaznah Y & Rahim MN)
SLE in Malaysian males: analysis of clinical, serological features and HLA types (Azizah MR, Ainol SS, Kuak SH, Kong NCT, Normaznah Y & Rahim MN)
The association of the HLA Class II antigens with clinical and autoantibody expression in Malaysian Chinese patients with Systemic Lupus Erythematosus (Azizah MR, Ainol SS, Kuak SH, Kong NCT, Normaznah Y & Rahim MN)
HLA antigens in Malay patients with Systemic Lupus Erythematosus: association with clinical and autoantibody expression (Azizah MR, Ainol SS, Kuak SH, Kong NCT, Normaznah Y & Rahim MN)
Construction of cDNA library and expressed sequence tags (ESTs) of local isolate of Giardia duodenalis (Latifah I, Teoh KY , Normaznah Y, Wan KL & Rahmah M)
Detection of Giardia lamblia isolate from human using PCR-ELISA (Latifah I, Teoh KY , Normaznah Y, Wan KL & Rahmah M)
Year 1999
Development of diagnostic system for the detection of Acanthamoeda castellanii species and characterization of the isolates from individuals with eye keratitis using different approaches of polymerase chain reaction (Noor Rain A, Rosmillah M, Rohaya C & Normaznah Y)
Monoclonal based-ELISA for the detection of Legionella pneumophila antigen in urine (Normaznah Y, Saniah K & Norazah A)
Detection of Legionella DNA in human urine samples by polymerase chain reaction (Normaznah Y, Saniah K & Norazah A)
Serum soluble interleukin-2 receptor levels in patients with systemic lupus erythematosus: correlation with clinical and laboratory parameters (Azizah MR, Ainol Shahera S, Rahim MN, Norella KCT & Normaznah Y)
Systemic lupus erythematosus in Malaysian men: clinical and immunological profile (Azizah MR, Ainol Shahera S, Rahim MN, Norella KCT & Normaznah Y)
Systemic lupus erythematosus: analysis of clinical and laboratory features (Azizah MR, Ainol Shahera S, Rahim MN, Norella KCT & Normaznah Y)
Construction of cDNA library of a local isolate of Giardia lamblia (Latifah I & Teoh KY )
Restriction fragment length polymorphism of Giardia lamblia by polymerase chain reaction (Latifah I, Teoh KY , Khoy DY & Normaznah Y)
Potential use of CD44 gene in diagnosis and screening of bladder cancer (Halim et al )
Year 1998
Evaluation of molecular variants of angiotensin-converting enzyme (ACE) gene as a genetic risk factor for coronary heart disease (CHD) in Malaysia (Normaznah Y, Norhatiah ML, Azizah MR, Noor Rain A & Rosli MA)
Protective immunity against Plasmodium berghei malaria after administration of Interleukin-12 (IL-12) (Normaznah et al )
Immunoblot analysis of adult somatic Fasciola gigantica antigens (Normaznah et al )
Allelic variants of the Plasmodium falciparum merozoite surface protein-1 gene (block 2) in Malaysian isolates (Noor Rain et al )
Development of diagnostic system for the detection of Acanthamoeba species and characterization of the isolates using different approaches of PCR (Noor Rain et al )
Antineutrophil cytoplasmic antibodies in patients with rheumatoid arthritis (Azizah MR et al )
HLA DR2 typing using PCR-RFLP (Azizah MR et al )
Determination of serologic indicators of clinical activity in patients with systemic lupus erythematosus (Azizah MR et al )
Tumour necrosis factor-alpha (TNF- a ) in human toxoplasmosis (Latifah I, Normaznah Y, Teoh KY , Azizah R & Thongkanh KS)
Potential anti-malarial activity of Eurycoma longifolia against Plasmodium berghei (Abdul Halim et al )
| Introduction | Research |
