Bioassay Unit

| Introduction | Research |

RESEARCH

  1. Toxicity study of Orthosiphon stamineus (Misai Kucing) in Sprague-Dawley rats. 2002-2005 (Dr. Noor Rain Abdullah ) (MOSTI)

  2. MMBPP Project Phase 2: Subproject 1: Advanced Research & Development on Tongkat Ali leading towards product discovery. Subsection F: Pharmacology study to analyse and validate anti-malaria propertise of Tongkat Ali. 2004 - 2005  (Dr. Noor Rain Abdullah ) (MOSTI)

  3. Mechanism of action, in vivo and ex-vivo bioassay study of medicinal plants: Goniothalamus scorthecinii (Selada putih, leaves), Aralidium pinnatifidium (Hempedu buaya, stem), Mitragynaspeciosa and Mitragynine (derivatives of M. speciosa) for anti malaria. August 2003 to August 2005  (Dr. Noor Rain Abdullah ) (IRPA)

  4. Toxicological studies of Hempedu Bumi (Andrographis paniculata) Project 2: Mutagenecity study of Hempedu Bumi (Andrographis paniculata) 2002 to 2004  (Murizal Zainol) MOSTI)

  5. Cellular cloning and genetic characterization of malaria parasite present in naturally occurring mixed population Mei 2005 to Mei 2006 (Mohd Ridzuan Mohd Abd. Razak ) (NIH)

  6. Development of cytotoxic component (SMD compound) from Eurycoma longifolia for anti anticancer agent (Shamsul Muhamad) (UKM)

  7. Development of carbonated-apatite material for possible biomadical implant proposes. (Shamsul Muhamad) (SIRIM)

  8. Effect of Eurycoma longifolia standardized extract on the glutathione (GSH) level of malaria infected erythrocyte: the role of redox metabolism in affecting the Plasmodium growth. (Mohd Ridzuan Mohd Abd. Razak) (IMR)

  9. Detection of Rat Cytocrome P450(2E1) using Western Blot Analysis and Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR).   ( Lailatul Jumaayah Salleh Huddin) (IMR)

  10. In vitro screening of 5 local medicinal plants for anti bacteria activity using disc diffusion method and MTT assay (Zaidan Rehtha Shafar ) (IMR)

  11. Anti proliferative activity of five medicinal plants with traditional claims as anti tumorals (Shamsul Muhamad) (IMR)

  12. “Drug Interaction : Interactions of Goniothalamine with Artemisinin and Chloroquine against P. berghei and P. yoelii in ICR mice and to P. falciparum in vitro”. (Mohd Ridzuan Mohd Abd. Razak) (DAP&E)

  13. Combination study of Eurycoma longifolia Standardized Extracts TA164 with chloroquine towards rodent malaria (Mohd Ridzuan Mohd Abd. Razak ) (IMR)

  14. Plasmodium falciparum Malaysian Clone: Genotyping Using Restriction Fragment Length Polymorphism-PCR. (Mohd Ridzuan Mohd Abd. Razak) (NIH)

  15. The Effect of Eurycoma longifolia Extract on Plasmodium falciparum Aspartic Hemoglobinases Level (Mohd Ridzuan Mohd Abd. Razak) (IMR)

 

ACTIVITIES

  1. Screening of medicinal plants for anti malaria properties using schizont maturation assay and Plasmodium Lactate Dehydrogenasse Assay (pLDH assay). The in vivo efficacy screening are being carried out using the 4 Day Test. There are 404 plant extracts in the Plant Extract Database (for malaria screening).

  2. Screening of medicinal plants for anti proliferative properties in vitro using the appropriate cell lines and its efficacy evaluation in animal model.

  3. Screening for toxicity potential in vitro towards normal cells using MTT assays, Cytotoxic Detection Kit (CDK), and SRB method.

  4. Carrying out research on medicinal plant products/herbal preparations for anti bacteria properties through in vitro biological screening using the standard Disc Diffusion Method and the MTT assay.


ACHIEVEMENT

Publications

  1. Siti Najila MJ, Noor Rain A, Mohamad Kamel,AG, Syed Zahir, SI, Khozirah, S, Lokman Hakim, S, Zakiah, I and Azizol, AK. (2002) .The screening of extracts from Goniothalamus scorthecinii, Aralidium pinnatifidium and Andrographis paniculata for anti malarial activity using the lactate dehydrogenase assay. Journal of Ethnopharmacology , 82: 239-242.

  2. Chan Kit Lam, Choo Chee Yan, Noor Rain Abdullah, Zakiah Ismail. (2004). Anti plasmodia activity of Eurycoma longifolia Jack using the lactate dehydrogenase assay of Plasmodium falciparum . Journal Of Ethnopharmacology, 92: 223 – 227.

  3. Mohd Ridzuan M.A.R., Noor Rain, A., Zhari, I. and Zakiah, I. 2005. Effect of Eurycoma longifolia Extract On The Glutathione Level in Plasmodium falciparum Infected Erythrocytes in vitro. Tropical Biomedicine 22: 155-163.

  4. Mohd Ridzuan Mohd Abd Razak, Ruenruetai Udonsom, Noor Rain Abdullah, Khozirah Shaari and Zakiah Ismail. 2005. Antimalarial properties of Goniothalamin in Combination with Chloroquine Against Plasmodium yoelii and Plasmodium berghei Growth in Mice. Tropical Biomedicine (in press).

Seminar and workshops

1. Organising Malaysia India Bilateral Program: Workshop in Techniques in Immunomodulatory screening of Herbal drug. 25th to 30th July 2005.

 

| Introduction | Research |