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1.         General Procedure For The Submission Of Specimen

 

            Each specimen must be collected in the appropriate container, which must be labelled with the patient's name and registration number. Every specimen must be accompanied by a completed request form. Some of the tests require an appointment by calling the Medical Officer (MO) in the Allergy and Immunology Research Centre(AIRC).  Some of the tests also require a control specimen. Please provide the appropriate control specimen required.

            Please refer to the tables for instruction on the type and amount of specimen required for each of the test performed by the AIRC.

 

2.         Specimens Collection and Transport

 

2.1.       Specimens For Immunological Investigations

No Appointment Required

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Examinations                  Specimen  Required             Container

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Anti-Sm                        }           Serum                          Plain tube

Anti-RNP                      }

Anti-SSA/Ro                  }           Adult:   2 ml

Anti-SSB/La                   }

Anti-Jo 1                      }           Children:  1 ml

Anti-Scl-70                    }

Anti-Gastric Parietal                -do-                              -do-

Anti-Mitochondrial                   -do-                              -do-

Anti-Smooth Muscle                 -do-                              -do-

Anti-Cardiolipin                       -do-                              -do-

Anti-Acetycholine receptor     -do-                              -do-

Anti-Neutrophil Cytoplasmic   -do-                              -do-

antibodies (ANCA)

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2.2.       Specimens For Special Immunological Investigations

Appointment Required

Please Contact The Mlt-In-Charge: Tel No: 03-40402359

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Examinations               Specimen required                  Container

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HLA typing Class 1       Recipient:   20 ml blood                      * Sterile silicon-

    (serology)                 Donor:        20 ml blood                      coated tube with

200 IU heparin

 

HLA typing Class          10 ml blood                             EDTA tube       

    I and II

    (molecular)

                       

HLA Cross-matching    Recipient:  5 ml blood             Plain tube

            Donor:       15 ml blood                       * Sterile silicon-

                        coated tube with

200 IU heparin

 

Mixed lymphocyte       Patient:  20 ml blood              *Universal sterile culture                       Donor:   20 ml blood               bottle with

Control I:  20 ml blood                        heparin

                                    Control II: 20 ml blood

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Note:  * Tubes will be provided by the AIRC, IMR

 

 

2.3.       Specimens For Special Immunological Investigations

Appointment Required

Please Contact The Mo-In-Charge: Tel No: 03-40402357/ 506

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Examinations               Specimen required                  Container

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IgA       }                       Adult:   2 ml serum                   Plain tube

IgM      }

IgG      }                       Child:   1 ml serum

 C3       }

 C4       }

Enumeration of                       Adult:   2.5 ml blood               EDTA tube

    T and B cells             Child:   2 ml blood

 

Phagocytic                  Patient: 2.5 ml blood              EDTA tube

Function Test                Control:  2.5 ml blood             EDTA tube

 

Lymphocytic                Patient:  10 ml blood              *Universal sterile Transformation                      Control: 10 ml blood               bottle with 0.02 Test                                                                            ml heparin

 

Allergy test:

Total Ig E                      3 ml blood                               Plain tube

Specific Ig E Classes     3-5 ml blood                            Plain tube

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Note:  * Tubes will be provided by the Division of Immunology IMR

 

 

1.       Normal Values

 

3.1.   Normal Values:   Immunoglobulins

 

 

Age

 

 

Ig G (mg/100ml)

 

Ig A (mg/100ml)

 

Ig M (mg/100ml)

Newborn

900 - 1500

0 - 5

5 - 20

1 - 3 months

250 - 550

5 - 50

20 - 40

4 - 6 months

300 - 600

10 - 50

30 - 60

7 - 12 months

400 - 900

20 - 60

35 - 75

2 years

550 - 1000

35 - 75

40 - 80

3 years

550 - 1100

50 - 110

40 - 85

4 - 5 years

550 - 1100

60 - 150

40 - 95

6 - 9 years

550 - 1200

60 - 170

40 - 95

12 years

550 - 1400

60 - 200

40 - 110

Adult

550 - 1500

60 - 320

45 - 145

 

 

3.2.       Normal Values:   Complements

 

C 3 level = 50 - 90 mg / 100 ml.

 

C 4 level = 10 - 40 mg / 100 ml.

3.3.       Normal Values:   Total Ig E Level

 

 

Age Group ( years )

 

 

Ig E Level  ( IU / ml )

0 - 3

< 10

3 - 4

< 25

4 - 7

< 50

7 - 14

< 100

15 and above

< 150

Average

27.6

 

 

3.4.       Normal Values:  T   And   B  Cells Enumeration

 

 

 

 

Cord Blood

 

1day –

11 months

 

1-6 years

 

7 –17 years

 

Adult

WBC

Absolute count

 

 

10,000-15,000

 

6,400-11,000

 

6,800-10,000

 

4,700-7,300

 

4.2 - 8.6 (x109 / L

Lymphocyte

Percentage

Absolute count

 

35 - 47

4,200-6,900

 

39 - 59

2,700-3,600

 

38 - 53

2,900-5,100

 

36 - 43

2,000-2,700

 

22 - 55

1,500-3,770

T cells

Percentage

Absolute count

 

49 - 62

2,400-3,700

 

58 - 67

1,700-3,600

 

62 - 69

1,800-3,600

 

66 - 76

1,400-2,000

 

53 - 80

988-3,912

B cells

Percentage

Absolute count

 

14 - 23

700-1,500

 

19 - 31

500-1,500

 

21 - 28

700-1,300

 

12 - 22

300-500

 

5 - 22

130-716

CD4+Th cells

Percentage

Absolute count

 

 

28 - 42

1,500-2,400

 

38 - 50

1,700-2,800

 

30 - 40

1,000-1,800

 

33 - 41

700-1,100

 

19 - 46

431-1,976

CD8+Ts cells

Percentage

Absolute count

 

26 - 33

1,200-2,000

 

18 - 25

800-1,200

 

25 - 32

800-1,500

 

27 - 35

600-900

 

17 - 49

385-1,805

NK cells

Percentage

Absolute count

 

14.0- 30.0

800-1,800

 

8.0 - 17.0

300-700

 

8.0 - 15.0

200-600

 

9.0 - 16.0

200-600

 

8 - 37

227-1,354

CD4/CD8

Ratio

 

 

0.8 - 1.8

 

1.5 - 2.9

 

1.0 - 1.6

 

1.1 - 1.4