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Human Diagnosis of Rabies

. Last Updated: 19 July 2017Hits: 422

  

Diagnosis of Rabies Virus Infections among Human

Diagnosis tests for rabies virus are available at the Virology Unit.

Diagnostics tests are indicated when patients start showing sign and symptoms especially CNS symptoms. 

 

The diagnostics tests include the following tests:

  1. Real time RT PCR for rabies 
  2. Direct Immunofluorescent test 
  3. Viral isolations using Vero and BHK cells 

 

rabies

Several samples required for diagnosis of rabies. The appropriate samples are:-

    1. Skin biopsy (hair follicle from nape of the neck)

      Obtain a full-thickness punch biopsy at least 5 to 6 mm in diameter and deep enough to include the cutaneous nerves at the base of hair follicles (include at least 10 hair follicles.)

  • Place on saline-soaked sterile gauze, and put into a sterile container
  • Keep at 40C
  • Without virus transport medium and preservative

 

     2. Saliva

  • At least 2-3ml
  • Put into a sterile container 
  • Keep at 40C
  • Without virus transport medium and preservative

 

    3. Urine

  • At least 5ml
  • Put into a sterile urine container 
  • Keep at 40C
  • Without virus transport medium and preservative

 

    4. Cerebral spinal fluid (CSF)

  • At least 2-3ml
  • Put into a sterile container
  • Keep at 40C
  • Without virus transport medium and preservative

 

    5. Serum/blood

  • At least 3-4ml
  • Put into a sterile container
  • Without virus transport medium and preservative

 

    6. Corneal scrapping

  • Corneal impression is done by an ophthalmologist by pressing the surface of a sterile glass slide gently but firmly onto the cornea.
  • Corneal scrapings is also done by an ophthalmologist where epithelial cells are gently collected using a sterile loop or spatula and smeared carefully on a glass slide.
  • Fixed in acetone

 

    7. Brain (Post mortem sample)

  • From Hippocampus/brain stem
  • At least 5-6mm
  • Put into a sterile container, moist with sterile saline 
  • Keep at 40C

 

 

 

All samples must be kept at 40C during transportation and send to IMR as soon as possible
Clinicians/Doctors are requested to consult first (03-26162671) before sending samples for rabies testing.

myGOV MAMPU JPA IKU MSC NMMR CRC MyHEALTH MOH Globinmed SPA krste.my

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